Evaluation of Antimicrobial Efficacy of Different Endodontic Sealers Against Root Canal Microorganisms: An In Vitro Study

Khajuria, Akriti; Gaur, Ankita; Pathania, Vasundhara; Sharma, Amit Kumar; Gupta, Kamal Kishor; Sood, Ishant

doi:10.14744/JPD.2024.10_149

Akriti Khajuria¹ , Ankita Gaur¹ , Vasundhara Pathania¹ , Amit Kumar Sharma¹ , Kamal Kishor Gupta¹ , Ishant Sood¹

¹Department of Pedodontics and Preventive Dentistry, Himachal Dental College, Sunder Nagar, India

Journal of Pediatric Dentistry 1-4 DOI: 10.14744/JPD.2024.10_149

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Abstract

Objective: The present study aimed to evaluate the antimicrobial efficacy of endodontic sealers that are - MTA Fillapex, AH26, Apexit Plus, Zinc Oxide Eugenol (ZOE), and Zinc oxide (ZO) + Thyme oil against E. coli, P. aeruginouosa, S. aureus, and E. faecalis.
Materials and Methods: The antimicrobial tests were performed using the Agar diffusion Method. Microorganisms were inoculated with Muller Hinton Agar, and Five holes were punched and filled with respective sealers: MTA Fillapex (Group 1); AH26 (Group2); Apexit Plus (Group 3); ZOE (Group 4) and ZO+ Thyme oil (Group 5). These plates were then incubated, zone of inhibition was checked after 24 hours. The data was statistically analyzed using one-way ANOVA and Tukey’s Test (p<0.05).
Results: All the sealers showed some level of antimicrobial efficacy against test microorganisms with the highest antimicrobial activity exhibited by Zinc oxide + Thyme oil followed by AH26, MTA Fillapex, Apexit Plus, and least by ZOE.
Conclusion: Zinc oxide + Thyme oil exhibited the highest antimicrobial efficacy against all test microorganisms.

Keywords: Antimicrobial efficacy, endodontic sealers, pediatric endodontics, thyme oil, zinc oxide eugenol