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Evaluation of Effect of 0.2% Chlorhexidine, 0.2% Magnetized Chlorhexidine and Magnetized RO Water Mouth Rinse on Streptococcus Mutans in Children: A Prospective Cross-Sectional Study
1Department of Pediatric and Preventive Dentistry, Hitkarini Dental College and Hospital, Jabalpur, India
2Department of Orthodontics and Dentofacial Orthopedics, Hitkarini Dental College and Hospital, Jabalpur, India
Journal of Pediatric Dentistry 2021; 7(2): 65-71 DOI: 10.14744/JPD.2021.05_46
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Objective: To compare efficacy of commercially available 0.2% chlorhexidine mouth rinse, conventionally prepared magnetized water and 0.2% magnetized chlorhexidine on reduction of S. mutans colony count.
Materials and Methods: A cross-sectional prospective study was performed on a group of 30 children of age 11-12 years. The study was conducted over a period of 1 week. Sample was equally distributed amongst the 3 groups (n=10) depending upon type of mouth rinse usage viz; Group I: 0.2% chlorhexidine mouthwash, Group II: 72 hours magnetized RO water and Group III: 72 hours magnetized 0.2% chlorhexidine mouthwash. Each group was asked to use 10 mL of the mouth rinse for 3 minutes once in a day for a period of 7 days. Saliva samples were collected at baseline, 3rd day and 7th day. Microbiological analysis of saliva samples was done for streptococcus mutant count on the inoculated cultural plates. One-way ANOVA test was applied to know the difference between the groups, further Tukey’s test was performed for multiple comparison.
Results: There was a significant reduction in S. mutans count in plaque after 7 days from baseline in all the three groups. Mean colony forming units at day 7 was 181.60, 185.80, 149.80 for 0.2% chlorhexidine mouthwash, magnetized water and magnetized 0.2% chlorhexidine mouthwash group respectively. The difference seen is statistically significant with magnetized 0.2% chlorhexidine mouthwash showing increased reduction in bacterial colony count.
Conclusion: Magnetically activated 0.2% chlorhexidine mouth wash is more potent in reducing the colony count in comparison to 0.2% chlorhexidine and magnetized water. Magnetized water can also be used as an environmental friendly mouth wash alternative to 0.2% chlorhexidine because of its comparable antibacterial efficacy.